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bceA [2018-05-04 16:41:09]
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bceA [2018-05-04 16:41:09]

primary bacitracin resistance determinant, ABC transporter (ATP-binding protein) for the export of bacitracin, plectasin, mersacidin and actagardine
Locus
BSU30380
Isoelectric point
9.29
Molecular weight
28.08 kDa
Protein length
253 aa Sequence Blast
Gene length
759 bp Sequence Blast
Function
export of toxic peptides
Product
bacitracin ABC transporter (ATP-binding protein)
Essential
no
Synonyms
ytsC

Genomic Context

      
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Categories containing this gene/protein

Gene

Coordinates
3,111,327 → 3,112,088

Phenotypes of a mutant

  • 85-fold increased sensitivity to bacitracin PubMed
  • The protein

    Protein family

  • ABC transporter family (according to Swiss-Prot)
  • Effectors of protein activity

  • the activity of the BceA-BceB ABC transporter is inhibited by heptaprenyl pyrophosphate which accumulates in a ytpB mutant PubMed
  • Structure

  • 1L2T (MJ0796 from Methanocaldococcus jannaschii , 38% identity) PubMed
  • Localization

  • membrane associated (via BceB) PubMed
  • Expression and Regulation

    Operons

    Genes
    Description

    Regulatory mechanism

  • BceR: activation, PubMed, in BceR regulon
  • Regulation

  • induced in the presence of bacitracin, plectasin, mersacidin and actagardine, already at very low levels of bacitracin (BceR) PubMed
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    Biological materials

    Mutant

  • MGNA-A167 (ytsC::erm), available at the NBRP B. subtilis, Japan
  • BKE30380 (ΔbceA::erm trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTACGCAGTCTCCTTTA, downstream forward: _UP4_CAAGGCGTGTTAGGCGGGGT
  • BKK30380 (ΔbceA::kan trpC2) available at BGSC, PubMed, upstream reverse: _UP1_CATTTACGCAGTCTCCTTTA, downstream forward: _UP4_CAAGGCGTGTTAGGCGGGGT
  • Labs working on this gene/protein

  • Foglino M. PubMed
  • References

    Reviews

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    Original publications

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